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	Provedor de dados Biocell (1) BJMBR (1) Autor Bustamante,J.O. (1) Cavicchia,Juan Carlos (1) Fóscolo,Mabel (1) Guembe,Gustavo (1) Varanda,W.A. (1) Palavra-chave Nuclear envelope (2) Nucleocytoplasmic transport (2) Apoptosis (1) Cell nucleus (1) Corpus luteum (1) Gene activity (1) Gene expression (1) Ion channels (1) Macromolecular transport (1) Measurement (1) Nuclear pores (1) Patch-clamp (1) Transcriptional control (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 1998 (1) País Argentina (1) Brazil (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat: A freeze-fracture study Biocell Cavicchia,Juan Carlos; Guembe,Gustavo; Fóscolo,Mabel. In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free... Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Corpus luteum; Nuclear envelope; Nucleocytoplasmic transport. Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200004 Patch-clamp detection of macromolecular translocation along nuclear pores BJMBR Bustamante,J.O.; Varanda,W.A.. The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nuclear pores; Nuclear envelope; Cell nucleus; Nucleocytoplasmic transport; Macromolecular transport; Gene activity; Gene expression; Transcriptional control; Ion channels; Patch-clamp; Measurement. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003 Registros recuperados: 2 Primeira ... 1 ... Última

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